rabbit anti mouse il 23p19  (Novus Biologicals)

Journal: Nature communications

Article Title: Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation.

doi: 10.1038/s41467-018-03704-z

Figure Lengend Snippet: Fig. 3 Increased cytokine expression in N-WASP-null skin. a qRT-PCR analysis of indicated genes from back skin RNA of con and ko at indicated ages (n > = 3/3, two-tailed unpaired t-test). All normalized to GAPDH. b Representative toluidine blue staining for barrier defect in con and ko mice at indicated ages (P: E15.5 embryos used as positive controls; 2 days (2d) n: 6/5, 6 days(6d) n: 4/5, 3 independent experiments). c Representative immunofluorescence staining and quantification of IL-23p19 of con and ko back skin at indicated ages (n: 3/3, two-tailed paired t-test). Scale bar: 100 µm. d Representative sorting strategy of CD45+, α6H, and α6L cells from con and ko epidermal cells and qRT-PCR analysis of IL-23A and IL-17A expression in sorted cells (n: 3/ 3, mean ± SD, two-tailed unpaired t-test, 3 independent sortings). e Representative immunoblots and quantification of indicated proteins from epidermal lysates of 7–8-week old con and ko mice (n: 4/4, mean ± SD, two-tailed unpaired t-test). f Representative immunoblots and quantification of indicated proteins of co-precipitations of IL-23p19 with IL-12p40 from epidermal lysates of 7–8-week-old con and ko mice (n: 4/4, mean ± SD, two-tailed unpaired t- test; *p ≤0.05; **p ≤0.01; ***p ≤0.001)

Article Snippet: Rabbit anti-mouse IL-23p19 (NBP1-77257, Novus, USA, 1:1000 dilution) and rat anti-mouse IL-12p40 (505201, BioLegend, USA, 1:100 dilution) were used for detection.

Techniques: Expressing, Quantitative RT-PCR, Two Tailed Test, Staining, Western Blot

Journal: Nature communications

Article Title: Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation.

doi: 10.1038/s41467-018-03704-z

Figure Lengend Snippet: Fig. 7 N-WASP represses IL-23p19 expression in keratinocytes by regulating H3K9 dimethylation. a, b Immunoblot analysis of H3K9me2 level in con and ko primary keratinocytes treated with BIX01294 (a) or IOX-1 (b) for 24 h (n: 3/3, mean ± SD, one-way ANOVA, with Tukey’s multiple comparisons). c Immunoblot for H3K9me2 in epidermis of 6-day-old mice (n: 3/3, mean ± SD, two-tailed unpaired t-test). d H3K9me2 IF of back skin of 6-day-old mice (n: 3/3). Scale bar: 100 µm. e qRT-PCR analysis of IL-23A mRNA expression in sorted interfollicular epidermal keratinocytes from con and G9a knockout. All normalized to GAPDH (n: 4/4, mean ± SD, two-tailed unpaired t-test). f ChIP of primary keratinocytes for H3K9me2 with qPCR for indicated regions of IL- 23 promoter (n: 7/7, mean ± SD, two-tailed unpaired t-test). g, h Primary N-WASP fl/flcells lentivirally transduced with GFP or Cre-GFP were analyzed by western blot for indicated genes (g) and by qRT-PCR for IL-23A expression (h; n: 4/4, mean ± SD, two-tailed unpaired t-test; *p ≤0.05; **p ≤0.01; ***p ≤0.001)

Article Snippet: Rabbit anti-mouse IL-23p19 (NBP1-77257, Novus, USA, 1:1000 dilution) and rat anti-mouse IL-12p40 (505201, BioLegend, USA, 1:100 dilution) were used for detection.

Techniques: Expressing, Western Blot, Two Tailed Test, Quantitative RT-PCR, Knock-Out, Transduction

Journal: Nature communications

Article Title: Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation.

doi: 10.1038/s41467-018-03704-z

Figure Lengend Snippet: Fig. 10 Decreased H3K9me2 in psoriatic lesions correlating with increased IL-23p19 expression. a, b H3K9me2 staining (a) and quantification (b) in healthy skin, non-lesioned and lesioned psoriatic skin (n: 3/3, mean ± SD, one-way ANOVA, with Tukey’s multiple comparisons). c H3K9me2 and IL-23p19 staining in non-lesioned and lesioned psoriatic skin (n: 3/3). Scale bars: 100 µm (a, c). (*p ≤= 0.05; **p ≤= 0.01)

Article Snippet: Rabbit anti-mouse IL-23p19 (NBP1-77257, Novus, USA, 1:1000 dilution) and rat anti-mouse IL-12p40 (505201, BioLegend, USA, 1:100 dilution) were used for detection.

Techniques: Expressing, Staining